Polyphenols from Ocimum basilicum improve lipoprotein metabolism: study of the activation of nuclear receptor (PPARs, LXRs, FXR
S Amrani
Department of Biology
Laboratory of Biochemistry
Faculty of Sciences,
University Mohamed First
Oujda, Morocco
Abstract:
In this work we demonstrated the effect of bioactive compounds extracted from sweet basil (Ocimum basilicum) on lipid metabolism. We assessed the effect of aqueous extract, fractions obtained by liquid-liquid extraction and fractions prepared by preparative HPLC. We found that these substances significantly reduce the elevated rate of plasma total cholesterol, triglycerides and LDL-cholesterol in mice treated with Triton either at 7 H or at 24 H. Our results showed also a significant reduction in atherogenic index and LDL/HDL-C ratio. On the other hand sweet basil improve lipid metabolism in hyperlipidemic mice fed with hyperlipidemic diet during 9 weeks. We demonstrated that the hypolipemic effect of basil bioactive substances is mediated by the induction of nuclear receptors involved in lipid metabolism and the activation of Apo A5 gene expression. However, we showed that the effect observed was not associated with the expression of Apo C-III and ABCA1 genes. In addition, our result demonstrates also that the observed hypolipidemic activity is partially mediated by the attenuation of cholesterol biosynthesis. The chromatographic analysis of bioactive substances of Ocimum basilicum shows the presence of luteolol 7-glucoside, two cafeic acid derivatives and rutin.
